thp 1 cells elicited il 1β secretion Search Results


il 1β production  (ATCC)
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ATCC il 1β production
Il 1β Production, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hkb il1b thp 1 cells dr gong cheng n a hela cells  (InvivoGen)
96
InvivoGen hkb il1b thp 1 cells dr gong cheng n a hela cells
Hkb Il1b Thp 1 Cells Dr Gong Cheng N A Hela Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa kit  (Thermo Fisher)
90
Thermo Fisher elisa kit
FAC inhibited influenza A virus infection in vitro and in vivo. a A549 cells were infected with PR8 (MOI = 0.1) ± (with or without) FAC (100 μM). 24 h later, supernatants were subject to viral titer assay. b A549 cells were infected with PR8 (MOI = 0.1), treated with increasing doses of FAC (μM). 12 h later, viral RNA loading was analyzed through real-time PCR. c , d 6–8-week old female C57BL/6 mice were intranasally infected with 1 × LD 50 influenza A virus PR8 diluted in 25 μl PBS or PBS with FAC (15 mM) (each group, n = 9). Mice weight change ( c ) and survival rate ( d ) were recorded. Mice with relative weight lower than 75% were euthanatized and considered as death. P < 0.0001 in ( c ) (PBS versus FAC, 2-way ANOVA test). P = 0.0033 in ( d ) (PBS versus FAC, log-rank (Mantel-Cox) test). e – h Mice were infected as in ( c ) 25 μl PBS or PBS with FAC (15 mM) was intranasally delivered at day 2 post infection. Whole lung and bronchoalveolar lavage fluids (BALFs) of mice were collected at days 3 and 6. The viral RNA loading in lung was analyzed by real-time PCR ( e <t>).</t> <t>IL-1β,</t> IL-6, TNF-α, and CCL2 in the whole lung and BALF were analyzed via <t>ELISA</t> ( f ). Infiltrated total cells and neutrophils (Ly-6G + ) in BALFs were analyzed through flow cytometry ( g ). Lung tissue inflammation was visualized via H&E staining (left) ( h ), arrows indicate tissue damage. Ctrl, mice without infection or treatment; Scale bars, 200 μm. Histological scores (right) were made by estimating lung inflammation levels by a pathologist blinded to the study (0 = absent; 1 = light; 2 = moderate; 3 = severe). Data are representative of at least two independent experiments in ( a , b ). Error bars represent SEM in ( c ) or SD in other panels
Elisa Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
elisa kit - by Bioz Stars, 2026-03
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human il-1β elisa  (R&D Systems)
90
R&D Systems human il-1β elisa
FAC inhibited influenza A virus infection in vitro and in vivo. a A549 cells were infected with PR8 (MOI = 0.1) ± (with or without) FAC (100 μM). 24 h later, supernatants were subject to viral titer assay. b A549 cells were infected with PR8 (MOI = 0.1), treated with increasing doses of FAC (μM). 12 h later, viral RNA loading was analyzed through real-time PCR. c , d 6–8-week old female C57BL/6 mice were intranasally infected with 1 × LD 50 influenza A virus PR8 diluted in 25 μl PBS or PBS with FAC (15 mM) (each group, n = 9). Mice weight change ( c ) and survival rate ( d ) were recorded. Mice with relative weight lower than 75% were euthanatized and considered as death. P < 0.0001 in ( c ) (PBS versus FAC, 2-way ANOVA test). P = 0.0033 in ( d ) (PBS versus FAC, log-rank (Mantel-Cox) test). e – h Mice were infected as in ( c ) 25 μl PBS or PBS with FAC (15 mM) was intranasally delivered at day 2 post infection. Whole lung and bronchoalveolar lavage fluids (BALFs) of mice were collected at days 3 and 6. The viral RNA loading in lung was analyzed by real-time PCR ( e <t>).</t> <t>IL-1β,</t> IL-6, TNF-α, and CCL2 in the whole lung and BALF were analyzed via <t>ELISA</t> ( f ). Infiltrated total cells and neutrophils (Ly-6G + ) in BALFs were analyzed through flow cytometry ( g ). Lung tissue inflammation was visualized via H&E staining (left) ( h ), arrows indicate tissue damage. Ctrl, mice without infection or treatment; Scale bars, 200 μm. Histological scores (right) were made by estimating lung inflammation levels by a pathologist blinded to the study (0 = absent; 1 = light; 2 = moderate; 3 = severe). Data are representative of at least two independent experiments in ( a , b ). Error bars represent SEM in ( c ) or SD in other panels
Human Il 1β Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human il-1β elisa/product/R&D Systems
Average 90 stars, based on 1 article reviews
human il-1β elisa - by Bioz Stars, 2026-03
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elisa kit (88–7261)  (Thermo Fisher)
90
Thermo Fisher elisa kit (88–7261)
FAC inhibited influenza A virus infection in vitro and in vivo. a A549 cells were infected with PR8 (MOI = 0.1) ± (with or without) FAC (100 μM). 24 h later, supernatants were subject to viral titer assay. b A549 cells were infected with PR8 (MOI = 0.1), treated with increasing doses of FAC (μM). 12 h later, viral RNA loading was analyzed through real-time PCR. c , d 6–8-week old female C57BL/6 mice were intranasally infected with 1 × LD 50 influenza A virus PR8 diluted in 25 μl PBS or PBS with FAC (15 mM) (each group, n = 9). Mice weight change ( c ) and survival rate ( d ) were recorded. Mice with relative weight lower than 75% were euthanatized and considered as death. P < 0.0001 in ( c ) (PBS versus FAC, 2-way ANOVA test). P = 0.0033 in ( d ) (PBS versus FAC, log-rank (Mantel-Cox) test). e – h Mice were infected as in ( c ) 25 μl PBS or PBS with FAC (15 mM) was intranasally delivered at day 2 post infection. Whole lung and bronchoalveolar lavage fluids (BALFs) of mice were collected at days 3 and 6. The viral RNA loading in lung was analyzed by real-time PCR ( e <t>).</t> <t>IL-1β,</t> IL-6, TNF-α, and CCL2 in the whole lung and BALF were analyzed via <t>ELISA</t> ( f ). Infiltrated total cells and neutrophils (Ly-6G + ) in BALFs were analyzed through flow cytometry ( g ). Lung tissue inflammation was visualized via H&E staining (left) ( h ), arrows indicate tissue damage. Ctrl, mice without infection or treatment; Scale bars, 200 μm. Histological scores (right) were made by estimating lung inflammation levels by a pathologist blinded to the study (0 = absent; 1 = light; 2 = moderate; 3 = severe). Data are representative of at least two independent experiments in ( a , b ). Error bars represent SEM in ( c ) or SD in other panels
Elisa Kit (88–7261), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/elisa kit (88–7261)/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
elisa kit (88–7261) - by Bioz Stars, 2026-03
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hek-blue il-1β cells  (InvivoGen)
96
InvivoGen hek-blue il-1β cells
FAC inhibited influenza A virus infection in vitro and in vivo. a A549 cells were infected with PR8 (MOI = 0.1) ± (with or without) FAC (100 μM). 24 h later, supernatants were subject to viral titer assay. b A549 cells were infected with PR8 (MOI = 0.1), treated with increasing doses of FAC (μM). 12 h later, viral RNA loading was analyzed through real-time PCR. c , d 6–8-week old female C57BL/6 mice were intranasally infected with 1 × LD 50 influenza A virus PR8 diluted in 25 μl PBS or PBS with FAC (15 mM) (each group, n = 9). Mice weight change ( c ) and survival rate ( d ) were recorded. Mice with relative weight lower than 75% were euthanatized and considered as death. P < 0.0001 in ( c ) (PBS versus FAC, 2-way ANOVA test). P = 0.0033 in ( d ) (PBS versus FAC, log-rank (Mantel-Cox) test). e – h Mice were infected as in ( c ) 25 μl PBS or PBS with FAC (15 mM) was intranasally delivered at day 2 post infection. Whole lung and bronchoalveolar lavage fluids (BALFs) of mice were collected at days 3 and 6. The viral RNA loading in lung was analyzed by real-time PCR ( e <t>).</t> <t>IL-1β,</t> IL-6, TNF-α, and CCL2 in the whole lung and BALF were analyzed via <t>ELISA</t> ( f ). Infiltrated total cells and neutrophils (Ly-6G + ) in BALFs were analyzed through flow cytometry ( g ). Lung tissue inflammation was visualized via H&E staining (left) ( h ), arrows indicate tissue damage. Ctrl, mice without infection or treatment; Scale bars, 200 μm. Histological scores (right) were made by estimating lung inflammation levels by a pathologist blinded to the study (0 = absent; 1 = light; 2 = moderate; 3 = severe). Data are representative of at least two independent experiments in ( a , b ). Error bars represent SEM in ( c ) or SD in other panels
Hek Blue Il 1β Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa development kits  (PeproTech)
90
PeproTech elisa development kits
FAC inhibited influenza A virus infection in vitro and in vivo. a A549 cells were infected with PR8 (MOI = 0.1) ± (with or without) FAC (100 μM). 24 h later, supernatants were subject to viral titer assay. b A549 cells were infected with PR8 (MOI = 0.1), treated with increasing doses of FAC (μM). 12 h later, viral RNA loading was analyzed through real-time PCR. c , d 6–8-week old female C57BL/6 mice were intranasally infected with 1 × LD 50 influenza A virus PR8 diluted in 25 μl PBS or PBS with FAC (15 mM) (each group, n = 9). Mice weight change ( c ) and survival rate ( d ) were recorded. Mice with relative weight lower than 75% were euthanatized and considered as death. P < 0.0001 in ( c ) (PBS versus FAC, 2-way ANOVA test). P = 0.0033 in ( d ) (PBS versus FAC, log-rank (Mantel-Cox) test). e – h Mice were infected as in ( c ) 25 μl PBS or PBS with FAC (15 mM) was intranasally delivered at day 2 post infection. Whole lung and bronchoalveolar lavage fluids (BALFs) of mice were collected at days 3 and 6. The viral RNA loading in lung was analyzed by real-time PCR ( e <t>).</t> <t>IL-1β,</t> IL-6, TNF-α, and CCL2 in the whole lung and BALF were analyzed via <t>ELISA</t> ( f ). Infiltrated total cells and neutrophils (Ly-6G + ) in BALFs were analyzed through flow cytometry ( g ). Lung tissue inflammation was visualized via H&E staining (left) ( h ), arrows indicate tissue damage. Ctrl, mice without infection or treatment; Scale bars, 200 μm. Histological scores (right) were made by estimating lung inflammation levels by a pathologist blinded to the study (0 = absent; 1 = light; 2 = moderate; 3 = severe). Data are representative of at least two independent experiments in ( a , b ). Error bars represent SEM in ( c ) or SD in other panels
Elisa Development Kits, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/elisa development kits/product/PeproTech
Average 90 stars, based on 1 article reviews
elisa development kits - by Bioz Stars, 2026-03
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Image Search Results


FAC inhibited influenza A virus infection in vitro and in vivo. a A549 cells were infected with PR8 (MOI = 0.1) ± (with or without) FAC (100 μM). 24 h later, supernatants were subject to viral titer assay. b A549 cells were infected with PR8 (MOI = 0.1), treated with increasing doses of FAC (μM). 12 h later, viral RNA loading was analyzed through real-time PCR. c , d 6–8-week old female C57BL/6 mice were intranasally infected with 1 × LD 50 influenza A virus PR8 diluted in 25 μl PBS or PBS with FAC (15 mM) (each group, n = 9). Mice weight change ( c ) and survival rate ( d ) were recorded. Mice with relative weight lower than 75% were euthanatized and considered as death. P < 0.0001 in ( c ) (PBS versus FAC, 2-way ANOVA test). P = 0.0033 in ( d ) (PBS versus FAC, log-rank (Mantel-Cox) test). e – h Mice were infected as in ( c ) 25 μl PBS or PBS with FAC (15 mM) was intranasally delivered at day 2 post infection. Whole lung and bronchoalveolar lavage fluids (BALFs) of mice were collected at days 3 and 6. The viral RNA loading in lung was analyzed by real-time PCR ( e ). IL-1β, IL-6, TNF-α, and CCL2 in the whole lung and BALF were analyzed via ELISA ( f ). Infiltrated total cells and neutrophils (Ly-6G + ) in BALFs were analyzed through flow cytometry ( g ). Lung tissue inflammation was visualized via H&E staining (left) ( h ), arrows indicate tissue damage. Ctrl, mice without infection or treatment; Scale bars, 200 μm. Histological scores (right) were made by estimating lung inflammation levels by a pathologist blinded to the study (0 = absent; 1 = light; 2 = moderate; 3 = severe). Data are representative of at least two independent experiments in ( a , b ). Error bars represent SEM in ( c ) or SD in other panels

Journal: Cell Discovery

Article Title: Antiviral effects of ferric ammonium citrate

doi: 10.1038/s41421-018-0013-6

Figure Lengend Snippet: FAC inhibited influenza A virus infection in vitro and in vivo. a A549 cells were infected with PR8 (MOI = 0.1) ± (with or without) FAC (100 μM). 24 h later, supernatants were subject to viral titer assay. b A549 cells were infected with PR8 (MOI = 0.1), treated with increasing doses of FAC (μM). 12 h later, viral RNA loading was analyzed through real-time PCR. c , d 6–8-week old female C57BL/6 mice were intranasally infected with 1 × LD 50 influenza A virus PR8 diluted in 25 μl PBS or PBS with FAC (15 mM) (each group, n = 9). Mice weight change ( c ) and survival rate ( d ) were recorded. Mice with relative weight lower than 75% were euthanatized and considered as death. P < 0.0001 in ( c ) (PBS versus FAC, 2-way ANOVA test). P = 0.0033 in ( d ) (PBS versus FAC, log-rank (Mantel-Cox) test). e – h Mice were infected as in ( c ) 25 μl PBS or PBS with FAC (15 mM) was intranasally delivered at day 2 post infection. Whole lung and bronchoalveolar lavage fluids (BALFs) of mice were collected at days 3 and 6. The viral RNA loading in lung was analyzed by real-time PCR ( e ). IL-1β, IL-6, TNF-α, and CCL2 in the whole lung and BALF were analyzed via ELISA ( f ). Infiltrated total cells and neutrophils (Ly-6G + ) in BALFs were analyzed through flow cytometry ( g ). Lung tissue inflammation was visualized via H&E staining (left) ( h ), arrows indicate tissue damage. Ctrl, mice without infection or treatment; Scale bars, 200 μm. Histological scores (right) were made by estimating lung inflammation levels by a pathologist blinded to the study (0 = absent; 1 = light; 2 = moderate; 3 = severe). Data are representative of at least two independent experiments in ( a , b ). Error bars represent SEM in ( c ) or SD in other panels

Article Snippet: Human IL-1β in supernatants of THP-1 macrophages was quantified via eBiosciences ELISA kit (88–7261).

Techniques: Infection, In Vitro, In Vivo, Titer Assay, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Staining